Volume 36, Issue 3 (Journal of Advanced Materials-Fall 2017)
2017, 36(3): 87-99 |
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Kouhi M, Shamanian M, Fathi M, Prabhakaran M, Ramakrishna S. Poly (hydroxybutyrate co hydroxyvalerate) Nanofibrous Scaffold Containing HydroxyapatiteBredigite Nanoparticles: Characterization and Biological Evaluation. Journal of Advanced Materials in Engineering (Esteghlal) 2017; 36 (3) :87-99
URL: http://jame.iut.ac.ir/article-1-805-en.html
URL: http://jame.iut.ac.ir/article-1-805-en.html
1. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran. , monireh.kouhi@ma.iut.ac.ir
Abstract: (7023 Views)
In this work, poly (hydroxybutyrate co hydroxyvalerate) (PHBV) composite nanofibrous scaffold containing hydroxyapatite/bredigite (HABR) nanoparticles was fabricated through electrospining method. The morphology of prepared nanofibers and the state of the nanoparticles dispersion in nanofiber matrix were investigated using scanning and transmission electron microscopy, respectively. Evaluation of the mechanical properties of the nanofibrous scaffolds revealed that there is a limit to the nanoparticle concentration at which nanoparticles can improve the mechanical properties of the nanofibrous scaffolds. According to the results, PHBV/HABR nanofibers showed higher wettability compared to PHBV nanofibers. In vitro cell culture assay was done using human fetal osteoblast cells on nanofibrous scaffold. MTS assay revealed that cell proliferation on the composite nanofibrous scaffold was significantly higher than those on the pure scaffold after 10 and 15 days. Scanning electron microscopy- Energy dispersive X-ray spectroscopy and CMFDA colorimeter assay analysis showed that the cells on the PHBV/HABR scaffolds acquired higher mineral deposition than the cells on the pure PHBV and control sample scaffold. Based on the results we concluded that PHBV/HABR nanofibers scaffold with higher wettability, improved mechanical properties and cell behavior hold great potential in bone regeneration applications.
Keywords: Nanofibrous scaffold, Poly (hydroxybutirate co hydroxyvalerate), Hydroxyapatite, Bredigite, Bone regeneration.
Type of Study: Research |
Subject:
Biomaterials
Received: 2016/08/2 | Accepted: 2016/10/1 | Published: 2017/11/15
Received: 2016/08/2 | Accepted: 2016/10/1 | Published: 2017/11/15
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